Most Read Articles
3 days ago
Combining the DASH (Dietary Approaches to Stop Hypertension) diet with low sodium intake reduces systolic blood pressure (SBP) in individuals with pre- and stage 1 hypertension, with progressively higher reductions at greater levels of baseline SBP, a recent study has shown.
Tristan Manalac, Yesterday
Major depressive disorder (MDD) appears to be more prevalent in females than in males, particularly in those who are divorced or widowed, a recent study from Singapore has found.
6 days ago
Cognitive deficits in bipolar disorder are neurodevelopmental rather than neurodegenerative in nature, a recent study has reported. Furthermore, cognitive impairment in bipolar patients appears to be stable, in the majority at least.
2 days ago
Tadalafil may not be effective for reducing the decline in ambulatory ability in boys with Duchenne muscular dystrophy (DMD), as shown in a recent study.

10-gene, RNA-based signature detects circulating tumour cells from HCC

12 Mar 2017

A novel, multi-gene, RNA-based molecular signature can detect circulating tumour cells (CTCs) from hepatocellular carcinomas (HCCs) with high specificity and sensitivity, a new study claims.

A CTC-iChip microfluidic device was used to efficiently separate, and thus enrich, CTCs from hematopoietic cells from blood samples. RNA from the CTCs were extracted, amplified through digital PCR and compared against various pre-existing lists of gene transcripts, yielding a 10-gene signature to potentially identify CTCs from HCC.

This gene signature was tested against 26 healthy controls, 31 patients with chronic liver disease (CLD), 16 untreated HCC patients, 32 HCC patients receiving therapy, 15 HCC patients who had undergone curative interventions and have no evidence of the disease, and 44 patients with other malignancies.

The 10 genes identified were transferrin, retinol binding protein 4, glypican 3, fibrinogen gamma chain, fibrinogen beta chain, fatty acid binding protein 1, apolipoprotein H, albumin, alpha 2-HS glycoprotein and alphafetoprotein.

Of the patients with untreated HCC, 9 (56 percent) tested positive when evaluated using the gene signature. In contrast, only 1 patient (3 percent) with non-malignant CLDs and 2 controls (7.6 percent) tested positive (p<0.0001).

Similarly, only 1 patient (7 percent) with no evidence of the disease following curative interventions tested positive (p=0.56), indicating that the signature can identify patients with an active disease.

Furthermore, 6 of the 10 genes identified were able to distinguish between HCC and other malignancies. In fact, in patients with non-HCC malignancies, 39 cases (88 percent) were correctly differentiated from HCC at a sensitivity of 50 percent.

Broadly, the current study outlines a platform for detecting CTCs. Further developments can, in turn, apply this platform to other cancers.

Digital Edition
Asia's trusted medical magazine for healthcare professionals. Get your MIMS Doctor - Malaysia digital copy today!
DOWNLOAD
Editor's Recommendations
Most Read Articles
3 days ago
Combining the DASH (Dietary Approaches to Stop Hypertension) diet with low sodium intake reduces systolic blood pressure (SBP) in individuals with pre- and stage 1 hypertension, with progressively higher reductions at greater levels of baseline SBP, a recent study has shown.
Tristan Manalac, Yesterday
Major depressive disorder (MDD) appears to be more prevalent in females than in males, particularly in those who are divorced or widowed, a recent study from Singapore has found.
6 days ago
Cognitive deficits in bipolar disorder are neurodevelopmental rather than neurodegenerative in nature, a recent study has reported. Furthermore, cognitive impairment in bipolar patients appears to be stable, in the majority at least.
2 days ago
Tadalafil may not be effective for reducing the decline in ambulatory ability in boys with Duchenne muscular dystrophy (DMD), as shown in a recent study.