Most Read Articles
Elvira Manzano, 5 days ago
Bisphosphonates have proven antifracture efficacy and remain to be the cornerstone of osteoporosis treatment. However, a drug holiday is of particular importance with bisphosphonates due to some signals with long-term use of the drug, including rare incidence of atypical femoral fracture (AFF) and osteonecrosis of the jaw (ONJ), says a leading endocrinologist at AFOS 2017.
Yesterday
Breast cancer patients have notably different microbiomes in the local breast tissue and urinary tract, a recent study reveals. Particularly, species in the Methylobacterium genus are reduced in the local breast tissue while the urinary tract is enriched in gram-positive bacteria.
Pearl Toh, 13 Oct 2017
Women with higher plasma tryptophan concentrations were less likely to have poor sleep quality during pregnancy, especially among those with anxiety symptoms, according to the GUSTO* study.
Tristan Manalac, Yesterday
Statin use, particularly in smokers, obese patients and nondiabetics, has a strong and dose-dependent association with decreasing risk of pancreatic ductal adenocarcinoma (PDAC), according to a new study.

10-gene, RNA-based signature detects circulating tumour cells from HCC

12 Mar 2017

A novel, multi-gene, RNA-based molecular signature can detect circulating tumour cells (CTCs) from hepatocellular carcinomas (HCCs) with high specificity and sensitivity, a new study claims.

A CTC-iChip microfluidic device was used to efficiently separate, and thus enrich, CTCs from hematopoietic cells from blood samples. RNA from the CTCs were extracted, amplified through digital PCR and compared against various pre-existing lists of gene transcripts, yielding a 10-gene signature to potentially identify CTCs from HCC.

This gene signature was tested against 26 healthy controls, 31 patients with chronic liver disease (CLD), 16 untreated HCC patients, 32 HCC patients receiving therapy, 15 HCC patients who had undergone curative interventions and have no evidence of the disease, and 44 patients with other malignancies.

The 10 genes identified were transferrin, retinol binding protein 4, glypican 3, fibrinogen gamma chain, fibrinogen beta chain, fatty acid binding protein 1, apolipoprotein H, albumin, alpha 2-HS glycoprotein and alphafetoprotein.

Of the patients with untreated HCC, 9 (56 percent) tested positive when evaluated using the gene signature. In contrast, only 1 patient (3 percent) with non-malignant CLDs and 2 controls (7.6 percent) tested positive (p<0.0001).

Similarly, only 1 patient (7 percent) with no evidence of the disease following curative interventions tested positive (p=0.56), indicating that the signature can identify patients with an active disease.

Furthermore, 6 of the 10 genes identified were able to distinguish between HCC and other malignancies. In fact, in patients with non-HCC malignancies, 39 cases (88 percent) were correctly differentiated from HCC at a sensitivity of 50 percent.

Broadly, the current study outlines a platform for detecting CTCs. Further developments can, in turn, apply this platform to other cancers.

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Most Read Articles
Elvira Manzano, 5 days ago
Bisphosphonates have proven antifracture efficacy and remain to be the cornerstone of osteoporosis treatment. However, a drug holiday is of particular importance with bisphosphonates due to some signals with long-term use of the drug, including rare incidence of atypical femoral fracture (AFF) and osteonecrosis of the jaw (ONJ), says a leading endocrinologist at AFOS 2017.
Yesterday
Breast cancer patients have notably different microbiomes in the local breast tissue and urinary tract, a recent study reveals. Particularly, species in the Methylobacterium genus are reduced in the local breast tissue while the urinary tract is enriched in gram-positive bacteria.
Pearl Toh, 13 Oct 2017
Women with higher plasma tryptophan concentrations were less likely to have poor sleep quality during pregnancy, especially among those with anxiety symptoms, according to the GUSTO* study.
Tristan Manalac, Yesterday
Statin use, particularly in smokers, obese patients and nondiabetics, has a strong and dose-dependent association with decreasing risk of pancreatic ductal adenocarcinoma (PDAC), according to a new study.