Most Read Articles
18 days ago
Regardless of dosing, nonvitamin K antagonist oral anticoagulants (NOACs) are more effective than warfarin for atrial fibrillation and safer in Asian than in non-Asian populations, a new meta-analysis shows.
6 days ago
Nonalcoholic fatty liver diseases (NAFLD) and serum concentrations of alanine transaminase (ALT), aspartate transaminase (AST), gamma-glutamyltransferase (GGT) and alkaline phosphatase (ALP) are independent predictors of incident type 2 diabetes, a new prospective study shows.
5 days ago
There appears to be no evidence supporting associations between physical activity and asthma control level, spirometric measurements and quality of life in children and adolescents with asthma, according to a study from Brazil.
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There is a growing number of targeted therapies being approved for use in cancer therapy in the home setting, and this poses a potential risk among family member and caregivers, particularly those of reproductive risk age, a recent study suggests.

10-gene, RNA-based signature detects circulating tumour cells from HCC

4 months ago

A novel, multi-gene, RNA-based molecular signature can detect circulating tumour cells (CTCs) from hepatocellular carcinomas (HCCs) with high specificity and sensitivity, a new study claims.

A CTC-iChip microfluidic device was used to efficiently separate, and thus enrich, CTCs from hematopoietic cells from blood samples. RNA from the CTCs were extracted, amplified through digital PCR and compared against various pre-existing lists of gene transcripts, yielding a 10-gene signature to potentially identify CTCs from HCC.

This gene signature was tested against 26 healthy controls, 31 patients with chronic liver disease (CLD), 16 untreated HCC patients, 32 HCC patients receiving therapy, 15 HCC patients who had undergone curative interventions and have no evidence of the disease, and 44 patients with other malignancies.

The 10 genes identified were transferrin, retinol binding protein 4, glypican 3, fibrinogen gamma chain, fibrinogen beta chain, fatty acid binding protein 1, apolipoprotein H, albumin, alpha 2-HS glycoprotein and alphafetoprotein.

Of the patients with untreated HCC, 9 (56 percent) tested positive when evaluated using the gene signature. In contrast, only 1 patient (3 percent) with non-malignant CLDs and 2 controls (7.6 percent) tested positive (p<0.0001).

Similarly, only 1 patient (7 percent) with no evidence of the disease following curative interventions tested positive (p=0.56), indicating that the signature can identify patients with an active disease.

Furthermore, 6 of the 10 genes identified were able to distinguish between HCC and other malignancies. In fact, in patients with non-HCC malignancies, 39 cases (88 percent) were correctly differentiated from HCC at a sensitivity of 50 percent.

Broadly, the current study outlines a platform for detecting CTCs. Further developments can, in turn, apply this platform to other cancers.

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Most Read Articles
18 days ago
Regardless of dosing, nonvitamin K antagonist oral anticoagulants (NOACs) are more effective than warfarin for atrial fibrillation and safer in Asian than in non-Asian populations, a new meta-analysis shows.
6 days ago
Nonalcoholic fatty liver diseases (NAFLD) and serum concentrations of alanine transaminase (ALT), aspartate transaminase (AST), gamma-glutamyltransferase (GGT) and alkaline phosphatase (ALP) are independent predictors of incident type 2 diabetes, a new prospective study shows.
5 days ago
There appears to be no evidence supporting associations between physical activity and asthma control level, spirometric measurements and quality of life in children and adolescents with asthma, according to a study from Brazil.
5 days ago
There is a growing number of targeted therapies being approved for use in cancer therapy in the home setting, and this poses a potential risk among family member and caregivers, particularly those of reproductive risk age, a recent study suggests.